Knockin (KI) mouse service
Ozgene offers custom design and generation of knockin (KI) mice for your research needs.
Germline transmission has been achieved for all KI projects in the past 20 years with competitive timelines.
- Custom designed to your specifications
- Make specific change to the gene of interest
- Point mutations, human mutations, reporters
- Conditional KI recommended
- Non-conditional approach may cause lethality and deleterious phenotypes
Ozgene's full KI service includes
- Custom design and construction of gene targeting vector and probes
- Full sequencing of the vector and probes using Southern Blot Analysis (SBA)
- Electroporation of embryonic stem (ES) cells
- Screening, expansion and confirmation of gene targeted ES cell clones
- Injection of ES cells into blastocysts
- Generation and breeding of ES cell chimeras
- Genotyping of germline offspring
- Standard service ends with heterozygous targeted germline offspring
Additional breeding services
- Cre and FLPe breeding
- Reserve breeding stock
- Homzygous lines
- Expansion of lines
- Custom breeding
- Cyropreservation
Ozgene animals are maintained at Ozgene's Vivarium under Specific Pathogen Free (SPF) conditions, conforming with the requirements of major international research institutes. Ozgene has relevant national and international accreditations to house and export animals, such as OLAW, OGTR, AQIS and DLGRD.