Transgenic (tg) services
Ozgene offers custom design and generation of transgenic (tg) mice for your research needs.
Gene targeting approach is recommended over randomly integrated tg approaches.
- Designed to your specifications
- Overexpress your transgene
- Insert cDNA into specific loci
- Phenotype a single mouse line
- Predictable expression pattern
- Various breeding options
Ozgene's full transgenic service includes
- Sequencing and cloning of client cDNA into Ozgene's vector backbone
- Full sequencing of the vector and probes using Southern Blot Analysis (SBA)
- Electroporation of embryonic stem (ES) cells
- Screening, expansion and confirmation of gene targeted ES cell clones
- Injection of ES cells into blastocysts
- Generation and breeding of ES cell chimeras
- Genotyping of germline offspring
- Standard service ends with heterozygous targeted germline offspring
Additional breeding services
- Cre and FLPe breeding
- Reserve breeding stock
- Homzygous lines
- Expansion of lines
- Custom breeding
- Cyropreservation
Ozgene animals are maintained at Ozgene's Vivarium under Specific Pathogen Free (SPF) conditions, conforming with the requirements of major international research institutes. Ozgene has relevant national and international accreditations to house and export animals, such as OLAW, OGTR, AQIS and DLGRD.