Immunity 1996 Apr;4(4):407
Ferrero, E; Goyert, SM.; Haziot, A; Hijiya, N; Kontgen, F; Silver, J; Stewart, CL; Yamamoto, S
Division of Molecular Medicine, North Shore University Hospital, Cornell University Medical College, Manhasset, New York 11030, USA.
Endotoxin shock is the result of activation of the immune system by endotoxin/LPS, a component of Gram-negative bacteria. CD14, a GPI-anchored glycoprotein expressed strongly by monocyte/macrophages, is one of several receptors for endotoxin/LPS. The role of CD14 in bacterial-induced and LPS-induced shock was tested in CD14-deficient mice produced by gene targeting in embryonic stem cells. CD14-deficient mice were found to be highly resistant to shock induced by either live Gram-negative bacteria or LPS; however, at very high concentrations of LPS or bacteria, responses through non-CD14 receptors could be detected. Surprisingly, CD14-deficient mice also showed dramatically reduced levels of bacteremia, suggesting an unexpected role for CD14 in the dissemination of Gram-negative bacteria.