Mediators of inflammation 2013;2013:953841. doi: 10.1155/2013/953841. Epub 2013 Dec 15.
Rodríguez-Gallego, E; Riera-Borrull, M; Hernández-Aguilera, A; Mariné-Casadó, R; Rull, A; Beltrán-Debón, R; Luciano-Mateo, F; Menendez, JA; Vazquez-Martin, A; Sirvent, JJ; Martín-Paredero, V; Corbí, AL; Sierra-Filardi, E; Aragonès, G; García-Heredia, A; Camps, J; Alonso-Villaverde, C; Joven, J
Unitat de Recerca Biomèdica, Hospital Universitari Sant Joan, Institut d'Investigació Sanitària Pere Virgili, Universitat Rovira i Virgili, Carrer Sant Llorenç 21, 43201 Reus, Spain ; Campus of International Excellence Southern Catalonia, Spain. Catalan I
Excessive energy management leads to low-grade, chronic inflammation, which is a significant factor predicting noncommunicable diseases. In turn, inflammation, oxidation, and metabolism are associated with the course of these diseases; mitochondrial dysfunction seems to be at the crossroads of mutual relationships. The migration of immune cells during inflammation is governed by the interaction between chemokines and chemokine receptors. Chemokines, especially C-C-chemokine ligand 2 (CCL2), have a variety of additional functions that are involved in the maintenance of normal metabolism. It is our hypothesis that a ubiquitous and continuous secretion of CCL2 may represent an animal model of low-grade chronic inflammation that, in the presence of an energy surplus, could help to ascertain the afore-mentioned relationships and/or to search for specific therapeutic approaches. Here, we present preliminary data on a mouse model created by using targeted gene knock-in technology to integrate an additional copy of the CCl2 gene in the Gt(ROSA)26Sor locus of the mouse genome via homologous recombination in embryonic stem cells. Short-term dietary manipulations were assessed and the findings include metabolic disturbances, premature death, and the manipulation of macrophage plasticity and autophagy. These results raise a number of mechanistic questions for future study.