What more can Ozgene offer you?
Once your new mouse line has been generated, you can utilise the convenient additional breeding options here at Ozgene. Our Vivarium provides the perfect site for your mouse breeding needs. It is Specific Pathogen Free (SPF) and complies with all major national and international research institutes.
We use our Geneoz VMS web-based management platform to manage all mice at our facility, ensuring effective and efficient breeding services for your project.
All breeding is customised to your project needs. Please see a list of additional breeding services below or contact us for a customised breeding plan.
Cre breeding >
Cre knockin mice are bred to your gene targeted mice. The Cre gene is expressed early in embryonic development resulting in the removal of floxed sequences. We can also breed your mice to remove the Cre gene. Your gene targeted mice that carry the Cre gene will be mated with wild-type mice of the appropriate genetic background. Gene targeted offspring lacking the Cre gene will be determined by Southern Blot Analysis.
Flp breeding >Flp mice are bred to your gene targeted mice. The Flp gene is expressed early in embryonic development resulting in the removal of sequences flanked by FRT sites. It is also possible to remove the Flp gene from the genome of your gene targeted mice. Your gene targeted mice that carry the Flp gene will be mated with wild-type mice of the appropriate genetic background. Gene targeted offspring lacking the Flp gene will be determined by Southern Blot Analysis.
Tissue specific breeding >Many tissue specific Cre strains have been published and are available from various sources, most commonly The Jackson Laboratory. They can be imported on your behalf for mating to your gene targeted mice. Gene targeted offspring carrying the tissue specific Cre gene will be determined by Southern Blot Analysis.
Reserve breeding stock >Reserve breeders are generated from within a small breeding colony from your mice of choice. This service provides you with a backup and therefore, peace of mind whilst your mice are being shipped or you are establishing a breeding colony in your own facility. This ensures that breeding age mice are available to you at any time.
Homozygous lines >We will generate homozygous mice in which both alleles are genetically modified. This is recommended in cases where heterozygous gene targeted modification does not result in an obvious or observable phenotype due to the presence of the remaining wild-type allele. We will breed your heterozygous gene targeted male and female mice to generate offspring homozygous for the gene targeted modification. Offspring are then genotyped by Southern Blot Analysis. You can expect that 25% of offspring to be homozygous, 50% to be heterozygous and 25% to be wild-type.
Colony Production >We can establish and maintain a colony at a predetermined size, age and gender, made available for regular shipments. This production colony will be designed to specifically suit your requirements for one or more colonies.
Custom breeding >Custom breeding is exactly that, customised to your needs. It could include, for example, transferring your gene targeted locus onto another strain background over several generations. This service is usually discussed on a project by project basis.
To find out more about these exciting services