Cell Death Differ. 2016 Jan 15. doi: 10.1038/cdd.2015.161. [Epub ahead of print]
Tian, L; Choi, SC; Lee, HN; Murakami, Y; Qi, CF; Sengottuvelu, M; Voss, O; Krzewski, K; Coligan, JE
Receptor Cell Biology Section, Laboratory of Immunogenetics, NIAID, NIH: Pathology Core, Laboratory of Immunogenetics, NIAID, NIH, Rockville, MD, USA.
Homeostasis requires the immunologically silent clearance of apoptotic cells before they become pro-inflammatory necrotic cells. CD300f (CLM-1) is a phosphatidylserine receptor known to positively regulate efferocytosis by macrophages, and CD300f gene-deficient mice are predisposed to develop a lupus-like disease. Here we show that, in contrast to CD300f function in macrophages, its expression inhibits efferocytosis by DC, and its deficiency leads to enhanced antigen processing and T-cell priming by these DC. The consequences are the expansion of memory T cells and increased ANA levels in aged CD300f-deficient mice, which predispose CD300f-deficient mice to develop an overt autoimmune disease when exposed to an overload of apoptotic cells, or an exacerbated autoimmunity when combined with FcγRIIB deficiency. Thus, our data demonstrates that CD300f helps to maintain immune homeostasis by promoting macrophage clearance of self-antigens, while conversely inhibiting DC uptake and presentation of self-antigens.
The team at Ozgene has over two decades of experience creating customised knockout and knock-in mice for pivotal medical research globally. Over 350 scientific publications are based on research using Ozgene mice.