Publication in detail

Cell Rep. 2016 Aug 9;16(6):1664-76. doi: 10.1016/j.celrep.2016.07.005. Epub 2016 Jul 28.

Nucleolin-Mediated RNA Localization Regulates Neuron Growth and Cycling Cell Size.

Perry, RB; Rishal, I; Doron-Mandel, E; Kalinski, AL; Medzihradszky, KF; Terenzio, M; Alber, S; Koley, S; Lin, A; Rozenbaum, M; Yudin, D; Sahoo, PK; Gomes, C; Shinder, V; Geraisy, W; Huebner, EA; Woolf, CJ; Yaron, A; Burlingame, AL; Twiss, JL; Fainzilber, M

Weizmann Institute of Science, Rehovot 76100, Israel. University of South Carolina, Columbia, SC 29208; University of California, San Francisco, CA 94158; Boston Children's Hospital and Harvard Medical School, MA 02115, USA.

How can cells sense their own size to coordinate biosynthesis and metabolism with their growth needs? We recently proposed a motor-dependent bidirectional transport mechanism for axon length and cell size sensing, but the nature of the motor-transported size signals remained elusive. Here, we show that motor-dependent mRNA localization regulates neuronal growth and cycling cell size. We found that the RNA-binding protein nucleolin is associated with importin β1 mRNA in axons. Perturbation of nucleolin association with kinesins reduces its levels in axons, with a concomitant reduction in axonal importin β1 mRNA and protein levels. Strikingly, subcellular sequestration of nucleolin or importin β1 enhances axonal growth and causes a subcellular shift in protein synthesis. Similar findings were obtained in fibroblasts. Thus, subcellular mRNA localization regulates size and growth in both neurons and cycling cells.

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