Publication in detail

PLoS One. 2017 Dec 11;12(12):e0187959. doi: 10.1371/journal.pone.0187959. eCollection 2017.

Generation and phenotypic characterisation of a cytochrome P450 4x1 knockout mouse.

Kharkwal, H; Batool, F; Koentgen, F; Bell, DR; Kendall, DA; Ebling, FJP; Duce, IR

School of Life Sciences, University of Nottingham, Nottingham, United Kingdom. Department of Biochemistry, University of Karachi, Karachi, Pakistan. Ozgene Pty Ltd., Bentley DC, Western Australia, Australia. European Chemicals Agency, Helsinki, Finland.

Cytochrome P450 4x1 (Cyp4x1) is expressed at very high levels in the brain but the function of this protein is unknown. It has been hypothesised to regulate metabolism of fatty acids and to affect the activity of endocannabinoid signalling systems, which are known to influence appetite and energy metabolism. The objective of the present investigation was to determine the impact of Cyp4x1 on body weight and energy metabolism by developing a line of transgenic Cyp4x1-knock out mice. Mice were developed with a global knock-out of the gene; the full-length RNA was undetectable, and mice were viable and fertile. Both male and female Cyp4x1-knock out mice gained significantly more body weight on normal lab chow diet compared to control flox mice on the same genetic background. At necropsy, Cyp4x1-knock out male mice had significantly greater intra-abdominal fat deposits (P

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