Publication in detail

Cell Metab. 2018 Oct 8. pii: S1550-4131(18)30578-3. doi: 10.1016/j.cmet.2018.09.013. [Epub ahead of print]

Protein Kinase C Epsilon Deletion in Adipose Tissue, but Not in Liver, Improves Glucose Tolerance.

Brandon, AE; Liao, BM; Diakanastasis, B; Parker, BL; Raddatz, K; McManus, SA; OReilly, L; Kimber, E; van der Kraan, AG; Hancock, D; Henstridge, DC; Meikle, PJ; Cooney, GJ; James, DE; Reibe, S; Febbraio, MA; Biden, TJ; Schmitz-Peiffer, C

Diabetes and Metabolism Division, Garvan Institute of Medical Research, Sydney, NSW 2010, Australia; School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia. Baker Heart and Diabetes Institute, Melbourne, VIC 3004, Australia. St Vincent's Clinical School, University of New South Wales, Sydney, NSW 2010, Australia.

Protein kinase C epsilon (PKCɛ) activation in the liver is proposed to inhibit insulin action through phosphorylation of the insulin receptor. Here, however, we demonstrated that global, but not liver-specific, deletion of PKCɛ in mice protected against diet-induced glucose intolerance and insulin resistance. Furthermore, PKCɛ-dependent alterations in insulin receptor phosphorylation were not detected. Adipose-tissue-specific knockout mice did exhibit improved glucose tolerance, but phosphoproteomics revealed no PKCɛ-dependent effect on the activation of insulin signaling pathways. Altered phosphorylation of adipocyte proteins associated with cell junctions and endosomes was associated with changes in hepatic expression of several genes linked to glucose homeostasis and lipid metabolism. The primary effect of PKCɛ on glucose homeostasis is, therefore, not exerted directly in the liver as currently posited, and PKCɛ activation in this tissue should be interpreted with caution. However, PKCɛ activity in adipose tissue modulates glucose tolerance and is involved in crosstalk with the liver.

» Online Version

Proven Track Record

The team at Ozgene has over two decades of experience creating customised knockout and knock-in mice for pivotal medical research globally. Over 400 scientific publications are based on research using Ozgene mice.

Go to Publications

Global Client Base

Ozgene generates genetically customised mice for researchers around the world. Ozgene mice can be found in 31 different countries on 5 continents from small academic institutions to multinational pharmaceutical companies.

See Client Papers

Lean Management

Ozgene is applying Lean Management principles to deliver the highest quality services and shortest lead times to our customers. The implementation of Lean Culture has already seen an improvement in our processes and timelines.

Read More