US 8202727 B2
This invention discloses a cloning system and more particularly a system for sequentially cloning a plurality of heterologous nucleic acid sequences to assemble a chimeric construct of interest. The cloning system employs a marker sequence, which confers an identifiable characteristic on host cells in which it is contained, to chaperone individual insert nucleic acid sequences into recipient constructs that do not comprise the marker sequence but comprise other nucleic acid sequences for inclusion in the chimeric construct. Recombinant constructs into which one or more insert nucleic acid sequences have been introduced with the chaperone marker sequence are isolated by introducing recombinant constructs into host cells and identifying hosts cells with the identifiable characteristic.
Publication type: Grant
Application number: US 11/914,519
PCT number: PCT/AU2006/000650
Publication date: Jun 19, 2012
Filing date: May 17, 2006
Priority date: May 17, 2005
Also published as: CA2608636A1, EP1882036A1, EP1882036A4, EP1882036B1, US20080268447, WO2006122354A1
Inventors: Frank Koentgen
Original Assignee: Ozgene Pty Ltd.
View on Google Patents