CA 2470527 A1
The present invention provides methods and compositions for the integration of a nucleotide sequence of interest into the genome. Specifically, the methods of the present invention comprise contacting a lentiviral vector with the plasma membrane of an oocyte, early stage embryo, or blastula under conditions that permit the entry of the viral vector and the subsequent integration of the nucleotide sequence of interest into the genome. The methods further comprise culturing the oocyte or embryo contacted with the lentiviral vector under conditions that allow for the formation of a pre-implantation embryo.
Subsequently, the pre-implantation embryo can be transferred into a recipient vertebrate where it is allowed to develop into at least one genetically modified animal. The methods and compositions of the invention thereby allow for the production of genetically modified animals, particularly, vertebrates, and particularly mammals.
Publication type: Application
Application number: CA 2470527
PCT number: PCT/US2002/041020
Publication date: Jul 24, 2003
Filing date: Dec 19, 2002
Priority date: Dec 21, 2001
Also published as: CN1633495A, EP1465981A2, EP1465981A4, US20030167500, WO2003059923A2, WO2003059923A3
Inventors: Ram Ramabhadran, Frank Koentgen, John Wakefield
Applicant: Tranzyme, Inc., Ram Ramabhadran, Frank Koentgen, John Wakefield, Ozgene Pty. Ltd.
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