2026
STAR Protoc 2026 Jun 19;7(2):104456. doi: 10.1016/j.xpro.2026.104456. Epub 2026 Apr 2.
Protocol for immunomagnetic enrichment of CD8 T cells from complex murine tissues
Department of Immunology, Sir Charles Gairdner Hospital and Path West Laboratory Medicine, Perth, WA 6009, Australia. Medical School, University of Western Australia, Perth, WA 6009, Australia.
Service type: Stock strains
Abstract
Efficient isolation of CD8 T cells is essential for accurately investigating their roles in immune regulation and disease pathogenesis. Here, we present a protocol for the isolation and enrichment of CD8 T cells by positive selection from complex murine tissues, including bone marrow (BM), liver, heart, and kidneys. We describe steps for tissue harvesting and processing into single-cell suspensions. We then detail procedures for immunomagnetic CD8 T cell enrichment and assessment of cell purity and viability by flow cytometry.
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