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2026

STAR Protoc 2026 Jun 16, DOI: 10.1016/j.xpro.2026.104436

Protocol for immunomagnetic enrichment of T cells from complex murine tissues

Ella Trolio, Lelinh Duong, Michaela Lucas, Amy Prosser

Medical School, University of Western Australia, Perth, WA 6009, Australia Department of Immunology, Sir Charles Gairdner Hospital and PathWest Laboratory Medicine, Perth, WA 6009, Australia

Service type: Stock strains

Abstract

T cells are the central effectors and regulators of the adaptive immune response. This protocol provides a step-by-step approach for isolating and enriching total T cells by negative selection from complex murine tissues, including bone marrow (BM), liver, heart, and kidneys. We describe steps for tissue harvesting, preparation of single-cell suspensions, and immunomagnetic enrichment. We then outline procedures for flow cytometric assessment of cell purity and viability. This protocol enables efficient recovery of high-quality T cells for reliable downstream analyses.