Science 2005 Apr 15;308(5720):41
Auden, A; Caddy, J; Cunningham, JM; Elias, PM; Ellis, S; Hislop, N; Holleran, WM; Jane, SM.; Kaur, P; Ting, SB; Uchida, Y; Wilanowski, T; Zhao, LL
Royal Melbourne Hospital Post Office, Grattan Street, Parkville, Victoria, Australia 3050.
The Drosophila cuticle is essential for maintaining the surface barrier defenses of the fly. Integral to cuticle resilience is the transcription factor grainy head, which regulates production of the enzyme required for covalent cross-linking of the cuticular structural components. We report that formation and maintenance of the epidermal barrier in mice are dependent on a mammalian homolog of grainy head, Grainy head-like 3. Mice lacking this factor display defective skin barrier function and deficient wound repair, accompanied by reduced expression of transglutaminase 1, the key enzyme involved in cross-linking the structural components of the superficial epidermis. These findings suggest that the functional mechanisms involving protein cross-linking that maintain the epidermal barrier and induce tissue repair are conserved across 700 million years of evolution.
The team at Ozgene has over two decades of experience creating customised knockout and knock-in mice for pivotal medical research globally. Over 300 scientific publications are based on research using Ozgene mice.