Phenotyping request

Ozgene project or gene name*

AIM - What would you like to achieve?

Validate the genetic modification
Check additional biological results of the modification

TARGET - What to measure?

DNA
mRNA
Protein
Physical variables
Behaviour
Other (enter details in additional info)

PROJECT STAGE - When to measure?

ES cells
Mice

TECHNIQUE - How to measure?

DNA:   qPCR

mRNA:   RT-qPCR   MiSeq

Protein:   Microscopy   FACS   Western   ELISA   Other

Genotype and zygosity

Sex

Ages and/or timepoints

Tissues and/or conditions

Replicate numbers (technical/biological)

Controls

Preferred protocols

Preferred reagents/kits

Additional details which may help us design a phenotyping plan e.g. papers/methods for analysis, information on antibodies or gene expression data