ApoE-KO

ApoE-KO

ApoE-KO

Strain details
Nomenclature B6.129P2-Apoetm1UncJ/Ozarc
Common nameApoE KO
SynonymsB6.APOE, ApoE KO, B6.129P2-Apoetm1Unc/J
StrainCongenic, Targeted mutation
Coat colourBlack (a/a)
SpeciesMouse
JAX stock number002052
LocationArea Oz2
Weekly wean target30 males, 20 females

Strain description

  • MHC haplotype: H2Kb
  • Complement factor: C5 normal
  • Mice homozygous for the Apoetm1Unc mutation show a marked increase in total plasma cholesterol levels that are unaffected by age or sex.
  • Fatty streaks in the proximal aorta are found at 3 months of age. The lesions increase with age and progress to lesions with less lipid but more elongated cells, typical of a more advanced stage of pre-atherosclerotic lesion.
  • Moderately increased triglyceride levels have been reported in mice with this mutation on a mixed C57BL/6 x 129 genetic background.
  • Aged apoE-deficient mice (>17 months) have been shown to develop xanthomatous lesions in the brain consisting mostly of crystalline cholesterol clefts, lipid globules, and foam cells. Smaller xanthomas were seen in the choroid plexus and ventral fornix.
  • Recent studies indicate that apoE-deficient mice have altered responses to stress, impaired spatial learning and memory, altered long term potentiation, and synaptic damage.
  • Homozygous mice are viable and fertile.
  • The Apoetm1Uncmutant strain was developed in the laboratory of Dr. Nobuyo Maeda at The University of North Carolina at Chapel Hill to replace part of exon 3 and part of intron 3 of the apolipoprotein E (Apoe) gene replaced with a neomycin resistance (neo) cassette. The targeting vector was electroporated into 129P2/OlaHsd-derived E14Tg2a embryonic stem (ES) cells and resulting mice were backcrossed to C57BL/6J inbred mice for at least 10 generations.
  • This strain is transferred from ARC to Ozgene ARC in 2023.
  • ApoE KO mice are useful for studying cardiovascular disease, atherosclerosis and fat metabolism, as well as the role of ApoE in Alzheimer’s disease.

Past ARC and transfer reports:

Current Ozgene ARC reports (from 01-Jun-2023)

  • Mouse images are representative only. Actual phenotypes may vary based on genotype, sex, age, husbandry, health status, and other factors