B6-CD45.1

B6-CD45.1

B6-CD45.1

Strain details
Nomenclature B6.SJL-Ptprca Pepcb/BoyJ
Common nameB6 CD45.1
SynonymsPep Boy, Ptprca[a]J, PTP
StrainCongenic, Inbred
Coat colourBlack (a/a)
SpeciesMouse
JAX stock number002014
LocationArea Oz2

Strain descriptions

  • MHC haplotype: H2Kb
  • Complement factor: C5 normal
  • The B6 CD45.1 strain carries the Ptprca, commonly known as CD45.1 or Ly5.1. Ptprca (protein-tyrosine phosphatase, receptor type c) was the gene symbol designated to the CD45 antigen because of its homology with human placental protein-tyrosine phosphatase 1B. Ptprca is one of a family of protein-tyrosine phosphatase genes involved with the regulation of cell growth.
  • This strain is used widely in transplant studies because it carries the differential Ptprcapan leukocyte marker commonly known as CD45.1 or Ly5.1. Wild-type C57BL/6 inbred mice express the Ptprcb (CD45.2 or Ly5.2) allele.
  • B6 CD45.1 strain is known to exhibit congenital abnormalities, which include microphthalmia, corneal opacities, anophthalmia, hydrocephalus and portosystemic shunts.
  • Created by transferring the Ptprca and Pepcb genes of the inbred strain SJL/J onto the C57BL/6 background through serial backcrossing, and with selection at each generation for Ptprca by serotyping (Shen et al., 1985).
  • B6 CD45.1 mice are now one of the most widely used tools in adoptive transfer and mixed-bone-marrow chimera experiments because the transferred cells can be identified by using monoclonal antibodies specific to CD45.2 or CD45.1 (Chisolm et al. 2019).
  • Transplant studies, immunology research, cell growth regulation studies.

Past ARC and transfer reports:

Current Ozgene ARC reports (from 01-Jun-2024)

  • Mouse images are representative only. Actual phenotypes may vary based on genotype, sex, age, husbandry, health status, and other factors.
  • See www.jax.org for more B6 CD45.1strain information.
  • Chisolm DA, Cheng W, Colburn SA, Silva-Sanchez A, Meza-Perez S, Randall TD, Weinmann AS. Defining Genetic Variation in Widely Used Congenic and Backcrossed Mouse Models Reveals Varied Regulation of Genes Important for Immune Responses. Immunity. 2019 Jul 16;51(1):155-168.e5. doi: 10.1016/j.immuni.2019.05.006. Epub 2019 Jun 24. PMID: 31248780.