The B6 CD45.1 strain carries the Ptprca, commonly known as CD45.1 or Ly5.1. Ptprca (protein-tyrosine phosphatase, receptor type c) was the gene symbol designated to the CD45 antigen because of its homology with human placental protein-tyrosine phosphatase 1B. Ptprca is one of a family of protein-tyrosine phosphatase genes involved with the regulation of cell growth.
This strain is used widely in transplant studies because it carries the differential Ptprcapan leukocyte marker commonly known as CD45.1 or Ly5.1. Wild-type C57BL/6 inbred mice express the Ptprcb (CD45.2 or Ly5.2) allele.
B6 CD45.1 strain is known to exhibit congenital abnormalities, which include microphthalmia, corneal opacities, anophthalmia, hydrocephalus and portosystemic shunts.
Created by transferring the Ptprca and Pepcb genes of the inbred strain SJL/J onto the C57BL/6 background through serial backcrossing, and with selection at each generation for Ptprca by serotyping (Shen et al., 1985).
B6 CD45.1 mice are now one of the most widely used tools in adoptive transfer and mixed-bone-marrow chimera experiments because the transferred cells can be identified by using monoclonal antibodies specific to CD45.2 or CD45.1 (Chisolm et al. 2019).
These mice are sold subject to The Jackson Laboratory’s Conditions of Use and also the Australian Patent Number (2004268530). Information regarding conditions of use is available at: JAX General Terms and Conditions.
JAX mice are the gold standard for animal model research
Animals are not allowed to be used for propagation
See www.jax.org for more B6 CD45.1strain information.
Chisolm DA, Cheng W, Colburn SA, Silva-Sanchez A, Meza-Perez S, Randall TD, Weinmann AS. Defining Genetic Variation in Widely Used Congenic and Backcrossed Mouse Models Reveals Varied Regulation of Genes Important for Immune Responses. Immunity. 2019 Jul 16;51(1):155-168.e5. doi: 10.1016/j.immuni.2019.05.006. Epub 2019 Jun 24. PMID: 31248780.
