goGermline knockout & knock-in mice

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goGermline ISTT 3Rs prize

d03; 100% ES cell derived mice

d03; Published in Genesis

d03; Awarded ISTT 3Rs prize

"The potentially significant impact that the widespread application and use of this transgenic technology provides, makes it a worthy inaugural winner of the ISTT 3Rs prize, the aim of which is to raise awareness of the beneficial 3Rs impact that transgenic technologies can have in relation to the use of animals for scientific research."

- ISTT 3Rs Committee

What is goGermline?

goGermline is a fast and efficient way to generate knockout and knock-in mice. It is a revolutionary technology that incorporates the use of goGermline embryos for injecting embryonic stem (ES) cells. goGermline eliminates competition with “host” embryos, resulting in 100% ES cell derived offspring.

Ozgene is the only company that can guarantee that only ES cell derived animals are produced at chimera breeding. All of our knockout and knock-in projects use goGermline, with significant benefits to germline efficiency, project timelines and animal welfare. To experience the goGermline benefits firsthand, enquire about a mouse project with us or order goGermline embryos to trial them in your facility.

Watch the video to learn more about goGermline and its benefits to investigators and animal welfare.

Contact us to discuss your goGermline project or to order goGermline embryos.

Where’s the goGermline proof? >
At Ozgene, we are constantly striving towards improving our services. We devote a portion of our time to working on R&D projects. goGermline was one of these projects with an excellent outcome.

In 2014, we began seeing exciting results with goGermline. Several goGermline trials consistently replicated the results. See the attached PDF for offspring data. The goGermline data has also been published in a scientific publications:
- Koentgen et al. genesis. 2016 Mar 25. doi: 10.1002/dvg.22938.
- Zhou et al. genesis. 2019 Apr 19. doi: 10.1002/dvg.23295.
Germline transmission is achieved faster and more efficiently with no excess mice, expediting time to first heterozygotes and first experimental cohorts. It’s pretty exciting, 88% of the projects using goGermline reached germline transmission in the first month and the rest were achieved in the following month. This data was presented at the ISTT2019 meeting in Kobe, download poster here.

goGermline technology is being used for all of our client projects with consistent results.
How does goGermline benefit you? >
If a product or service does not benefit the client, then why offer it? Exactly! The following demonstrates the many benefits of this technology to you, our client.
1. generate: only ES cell derived mice
2. eliminate: all non-ES cell derived mice
3. reduce: time to generate knockout and knock-in mice
4. increase: germline efficiency, even with challenging ES cells
5. reduce: animal numbers
We do not want to keep this discovery just to ourselves. goGermline will also give Animal Ethics Committees an opportunity to improve their 3R’s and provides animal facilities with many cost and human benefits. Please see the attached PDF for a more comprehensive list of benefits.
How to utilise goGermline? >
1. License goGermline (worldwide patents published)
2. Undertake a full knockout or knock-in mouse project
3. Send us your own or repository ES cell lines to generate mice
Contact us now to discuss any of these options.

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Quick quote

ISTT2019 poster

Did you see the latest data on goGermline by Ozgene^® expediting time to first experimental cohorts? It’s pretty exciting, 88% of the projects using goGermline reached germline transmission in the first month and the rest were achieved in the following month.

Download poster

goGermline papers

Genesis. 2016 Jun;54(6):326-33. doi: 10.1002/dvg.22938.
Exclusive transmission of the embryonic stem cell-derived genome through the mouse germline. [read]
Koentgen F, Lin J, Katidou M, Chang I, Khan M, Watts J, Mombaerts P.

Genesis. 2019 Apr 19:e23295. doi: 10.1002/dvg.23295.
The testicular soma of Tsc22d3 knockout mice supports spermatogenesis and germline transmission from spermatogonial stem cell lines upon transplantation. [read]
Zhou, H; Zheng, Z; Koentgen, F; Khan, M; Mombaerts, P.

Proven Track Record

The team at Ozgene has over two decades of experience creating customised knockout and knock-in mice for pivotal medical research globally. Over 400 scientific publications are based on research using Ozgene mice.

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Global Client Base

Ozgene generates genetically customised mice for researchers around the world. Ozgene mice can be found in 31 different countries on 5 continents from small academic institutions to multinational pharmaceutical companies.

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Lean Management

Ozgene is applying Lean Management principles to deliver the highest quality services and shortest lead times to our customers. The implementation of Lean Culture has already seen an improvement in our processes and timelines.