NSG

NSG

NSG

Strain details
Nomenclature NOD.Cg-Prkdcscid ll2rgtm1Wjl /SzJ/Ozarc
Common nameNSG
SynonymsNOD-scid IL2Rgammanull, NOD-scid IL2Rgnull,NOD scid gamma
StrainCongenic, Spontaneous mutation, Targeted mutation
Coat colourAlbino (A/A Tyrc /Tyrc )
SpeciesMouse
Genetic backgroundNOD/ShiLtJ
JAX stock number005557
LocationArea Oz1

Strain descriptions

  • MHC haplotype: H2Kg7
  • NSG mice do not express the Prkdc gene nor the X-linked Il2rg gene.
  • The mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
  • NSG mice lack mature T cells, B cells, or functional NK cells, and are deficient in cytokine signaling, leading to better engraftment of human hematopoietic stem cells and peripheral-blood mononuclear cells than any other published mouse strain.
  • NSG mice exhibit a progressive hearing loss that is already severe by three months of age. Due to their level of immunodeficiency NSG mice need to be maintained in a ‘maximum barrier’.
  • This strain is only available for Australian and New Zealand researchers.
  • These double mutant mice were produced by breeding female NOD.CB17- Prkdcscid /J mice with male mice bearing the X-linked B6.129S4-Il2rgtm1Wjl /J allele. The resulting male mice heterozygous for the Prkdcscid allele and hemizygous for the Il2rgtm1Wjl allele were crossed to female NOD.CB17- Prkdcscid /J mice for eight generations.
  • Heterozygotes were interbred to produce mice homozygous for the Prkdcscid allele and homozygous (females) or hemizygous (males) for the Il2rgtm1Wjl allele.
  • NSG strain is imported from JAX into Animal Resource Centre (ARC) in 2021.
  • The stock strain is transferred to Ozgene ARC in 2023.
  • Recent publications have demonstrated this strain’s outstanding utility in the studies of islet transplantation, hematopoietic stem cells and cancer stem cells.

Past ARC and transfer reports:

Current Ozgene ARC reports (from 01-Jun-2023)

  • Mouse images are representative only. Actual phenotypes may vary based on genotype, sex, age, husbandry, health status, and other factors.