NSG
NSG
| Strain details | |
|---|---|
| Nomenclature | NOD.Cg-Prkdcscid ll2rgtm1Wjl /SzJ/Ozarc |
| Common name | NSG |
| Synonyms | NOD-scid IL2Rgammanull, NOD-scid IL2Rgnull,NOD scid gamma |
| Strain | Congenic, Spontaneous mutation, Targeted mutation |
| Coat colour | Albino (A/A Tyrc /Tyrc ) |
| Species | Mouse |
| Genetic background | NOD/ShiLtJ |
| JAX stock number | 005557 |
| Location | Area Oz1 |
| Weekly wean target | 100 males,100 females |
Strain descriptions
- MHC haplotype: H2Kg7
- NSG mice do not express the Prkdc gene nor the X-linked Il2rg gene.
- The mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
- NSG mice lack mature T cells, B cells, or functional NK cells, and are deficient in cytokine signaling, leading to better engraftment of human hematopoietic stem cells and peripheral-blood mononuclear cells than any other published mouse strain.
- NSG mice exhibit a progressive hearing loss that is already severe by three months of age. Due to their level of immunodeficiency NSG mice need to be maintained in a ‘maximum barrier’.
- This strain is only available for Australian and New Zealand researchers.
- These double mutant mice were produced by breeding female NOD.CB17- Prkdcscid /J mice with male mice bearing the X-linked B6.129S4-Il2rgtm1Wjl /J allele. The resulting male mice heterozygous for the Prkdcscid allele and hemizygous for the Il2rgtm1Wjl allele were crossed to female NOD.CB17- Prkdcscid /J mice for eight generations.
- Heterozygotes were interbred to produce mice homozygous for the Prkdcscid allele and homozygous (females) or hemizygous (males) for the Il2rgtm1Wjl allele.
- NSG strain is imported from JAX into Animal Resource Centre (ARC) in 2021.
- The stock strain is transferred to Ozgene ARC in 2023.
- Recent publications have demonstrated this strain’s outstanding utility in the studies of islet transplantation, hematopoietic stem cells and cancer stem cells.
Past ARC and transfer reports:
Current Ozgene ARC reports (from 01-Jun-2023)
- This strain is housed in Area Oz1
- Please see Oz1 health reports on our Biosecurity page
- Ozgene ARC terms and Conditions
- Subject to The Jackson Laboratory’s Conditions of Use and the Australian Patent Number (2004268530). Information regarding conditions of use is available at: JAX General Terms and Conditions
- This strain is subject to additional use restrictions:
- Notice regarding distribution of this strain to companies and/or for commercial use (#8).
- Use of MICE by companies or for-profit entities requires a no-fee JAX Leap License prior to shipping.
- JAX mice are the gold standard for animal model research
- Mouse images are representative only. Actual phenotypes may vary based on genotype, sex, age, husbandry, health status, and other factors.
- More info https://www.jax.org/strain/005557
