A multifunctional mouse model to study the role of Samd3

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Eur J Immunol. 2021 Oct 9. doi: 10.1002/eji.202149469. Online ahead of print.

A multifunctional mouse model to study the role of Samd3

Annika E Peters, Konrad Knöpper, Anika Grafen, Wolfgang Kastenmüller

Würzburg Institute of Systems Immunology, Max Planck Research Group at the Julius-Maximilians-Universität Würzburg, Wurzburg, Germany.

Service type: Knock-in mice


The capacity to develop immunological memory is a hallmark of the adaptive immune system. To investigate the role of Samd3 for cellular immune responses and memory development, we generated a conditional knock-out mouse including a fluorescent reporter and a huDTR cassette for conditional depletion of Samd3-expressing cells. Samd3 expression was observed in NK cells and CD8 T cells which are known for their specific function against intracellular pathogens like viruses. After acute viral infections, Samd3 expression was enriched within memory precursor cells and the frequency of Samd3-expressing cells increased during the progression into the memory phase. Similarly, during chronic viral infections, Samd3 expression was predominantly detected within precursors of exhausted CD8 T cells that are critical for viral control. At the functional level however, Samd3-deficient CD8 T cells were not compromised in the context of acute infection with Vaccinia virus or chronic infection with Lymphocytic choriomeningitis virus. Taken together, we describe a novel multifunctional mouse model to study the role of Samd3 and Samd3-expressing cells. We found that Samd3 is specifically expressed in NK cells, memory CD8 T cells and precursor exhausted T cells during viral infections, while the molecular function of this enigmatic gene remains further unresolved. This article is protected by copyright. All rights reserved. Keywords: CD8 T cell; NK cell; SAMD3; mouse model; viral infection.

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