Stem Cell Reports. 2022 Aug 1;S2213-6711(22)00364-2. doi: 10.1016/j.stemcr.2022.07.005. Online ahead of print.
Exclusive generation of rat spermatozoa in sterile mice utilizing blastocyst complementation with pluripotent stem cells
Functional Genomics Center Zurich, ETH Zurich and University of Zurich, Zurich 8057, Switzerland. Laboratory of Nutrition and Metabolic Epigenetics, Department of Health Sciences and Technology, ETH Zurich, Schwerzenbach 8603, Switzerland. ETH Phenomics Center, ETH Zurich, Zurich 8049, Switzerland. Laboratory of Regenerative and Movement Biology, Department of Health Sciences and Technology, ETH Zurich, Schwerzenbach 8603, Switzerland.
Service type: goGermline™ embryos
Blastocyst complementation denotes a technique that aims to generate organs, tissues, or cell types in animal chimeras via injection of pluripotent stem cells (PSCs) into genetically compromised blastocyst-stage embryos. Here, we report on successful complementation of the male germline in adult chimeras following injection of mouse or rat PSCs into mouse blastocysts carrying a mutation in Tsc22d3, an essential gene for spermatozoa production. Injection of mouse PSCs into Tsc22d3-Knockout (KO) blastocysts gave rise to intraspecies chimeras exclusively embodying PSC-derived functional spermatozoa. In addition, injection of rat embryonic stem cells (rESCs) into Tsc22d3-KO embryos produced interspecies mouse-rat chimeras solely harboring rat spermatids and spermatozoa capable of fertilizing oocytes. Furthermore, using single-cell RNA sequencing, we deconstructed rat spermatogenesis occurring in a mouse-rat chimera testis. Collectively, this study details a method for exclusive xenogeneic germ cell production in vivo, with implications that may extend to rat transgenesis, or endangered animal species conservation efforts.
Keywords: Blastocyst complementation; artificial reproductive technology; germ cell production; interspecies chimerism; pluripotency; sterility.