Rag1-KO

Rag1-KO

Rag1-KO

Strain details
Nomenclature B6.SVJ129-Rag1tm1Bal/Ozarc
Common nameRag1-KO
SynonymsBlack N10, RAG1 N10, RAG KO, Rag1 -/-
StrainMutant inbred
Coat ColourBlack
SpeciesMouse
Genetic backgroundC57BL/N10
LocationArea Oz1

Strain descriptions

  • MCH haplotype: H2K b
  • Complement factor: C5 normal
  • Rag1-deficient (Rag1-/-) mice are an immunodeficient stock strain that lack functional B and T lymphocytes.
  • Rag1 (recombination activation gene) is one of two activation genes for V(D)J recombination which occurs at seven unique loci including loci in mature B and T lymphocytes.
  • Mice that are homozygous for the Ragtm1Bal mutation express a non-leaky severe combined immune deficiency phenotype (Prkdcscid/Prkdcscid) and contain 15–130 times fewer cells in the thymus.
  • Thymocytes of Rag1-/- mice are larger than the heterozygous and wildtype equivalents and produce no CD3+ or T-cell receptor ab-positive (TCRab+) cells.
  • Homozygous Rag-1 deficient mice appear healthy and are indistinguishable from heterozygous and wildtype littermates despite being immunodeficient.
  • Both male and female homozygous mice are fertile and produce viable offspring.
  • Old mice can develop inclusion body nephropathy.
  • In order to accommodate their level of immunodeficiency, Rag1-deficient mice are maintained at Ozgene ARC in a maximum barrier environment, housed within individually ventilated cages (IVC).
  • Strain of Origin is 129S4/SvJae then backcrossed on C57BL/6J to N10 at ARC.
  • This stock strain is highly applicable to a range of autoimmune research fields.
  • Mouse images are representative only. Actual phenotypes may vary based on genotype, sex, age, husbandry, health status, and other factors.
  • Spanopoulou E, Roman CA, Corcoran LM, Schlissel MS, Silver DP, Nemazee D, Nussenzweig MC, Shinton SA, Hardy RR, Baltimore D. Functional immunoglobulin transgenes guide ordered B-cell differentiation in Rag-1-deficient mice. Genes Dev. 1994 May 1;8(9):1030-42. doi: 10.1101/gad.8.9.1030. PMID: 7926785.