Humanized mice

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humanized mice

Fast humanization with goGermline
Over 400 published mouse models
Reliable models, no off-target events

"Ozgene generated a humanized mouse model for our immune checkpoint target validation, which is responding perfectly in pre-clinical studies to validate candidate human therapeutic antibodies."

- Researcher in Drug Discovery, Biotechnology, Europe

Choose Ozgene for reliable humanized mice

Ozgene has a proven track record in generating humanized mice for medical research to study human diseases and therapeutics, including immune checkpoint target validation. Please see our publications and newsletter feature articles for examples of research results.

We use our published and patented goGermline technology in all of our mouse projects. goGermline allows the generation of humanized mice fast and efficiently while relying on the proven technique of gene targeting via homologous recombination. All of our mouse projects have resulted in germline transmission to date.

Ozgene is making continuous improvements on project timelines by stabilising, standardising and refining each individual process using Lean management. Our fastest gene targeting project was recently completed in 20 weeks and the current average timeline is 30 weeks.

  • What are humanized mice? >

    Humanized mice express human proteins, allowing in vivo studies on human conditions. In some cases it is possible to replace an entire mouse gene with its human counterpart. Sometimes it may be necessary to keep some mouse sequences to ensure that the humanized protein functions properly in the mouse.

  • How are humanized mice generated? >

    A human cDNA or human genomic DNA is homologously recombined into the mouse genome with the aim to replace the functional domain of the mouse gene with the human one. This leads to the expression of the human protein in place of the mouse protein. Each humanization project is based on your requirements and we will assess your gene of interest to find the most appropriate approach.

  • How does research benefit from humanized mice? >

    Humanized mice aim to recapitulate the in vivo function of human proteins. Therefore, they are widely used to study and develop treatments for human diseases. Humanized mice are particularly helpful for pre-clinical drug validation of human therapeutic antibodies. In recent years, they have become invaluable tools for cancer research as models for immune checkpoint target validation and immuno-oncology.

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Did you know?

Our published and patented goGermline technology utilises genetically sterile blastocysts to generate chimeras that produce 100% ES cell derived offspring. This leads to significant time savings and animal welfare improvements.

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KO & KI service

  • Design & construct vector & probes
  • Fully sequence vector & probes
  • Electroporate embryonic stem (ES) cells
  • Screen, expand & confirm ES cells
  • Inject ES cells into goGermline blastocysts
  • Generate & breed goGermline chimeras
  • Genotype germline offspring

Custom breeding

  • Cre breeding
  • FLPe breeding
  • Tissue specific breeding
  • Reserve breeding stock
  • Homozygous lines
  • Colony production
  • Customised breeding

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