Knock-in mice

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Knock-in mice
Faster projects with goGermline
Over 400 published mouse models
Breeding in Australia & USA

"I was impressed with the discussions with the Ozgene technical team, prior to us making a decision. Once the project started, regular updates made me feel like I knew what was going on at every step in the process."

- Prof. Deborah Henderson, Newcastle University, UK

Choose Ozgene for your knock-in mice

Our knock-in mouse projects now utilise goGermline, the revolutionary new technology to generate germline mice fast and efficiently. The knock-in mouse generation process can be tracked in real-time by logging into your myOzgene portal. All of our services, including breeding and phenotyping, are performed in-house, by our expert staff.

Ozgene is making continuous improvements on project timelines by stabilising, standardising and refining each individual process using Lean management. Ozgeneā€™s fastest gene targeting project was recently completed in 20 weeks and our current average project timeline is 30 weeks.

  • What are knock-in mice? >

    Knock-in mice have an insertion in a specific locus in order to produce genetically modified mice for customized research purposes. Reporter genes, human genes, related genes from the same organism, or point mutations can be introduced into the protein-coding region of a gene to produce knock-in mice.

  • What is Ozgene's method for producing knock-in mice? >

    We continue to rely on the well-published technique of gene targeting via homologous recombination to generate knock-in mice. The targeted modification is manipulated in embryonic stem (ES) cells in vitro. These manipulated ES cells are then used to generate knock-in mice with the desired modification. We recommend using a conditional knock-in approach (mini cDNA, Cre/lox inversion) to avoid deleterious outcomes that are often associated with non-conditional (constitutive) knock-in methods. This ensures controllable and inducible results with your knock-in mice.

  • Why generate conditional knock-in mice? >

    Our conditional knock-in mouse service eliminates risk that is often prevalent when a non-conditional (constitutive) knock-in method is used. Non-conditional knock-in mutations can cause ES cell lethality, chimera lethality and heterozygote lethality. They may also result in major disease phenotypes that impact on the breeding and maintenance of the newly created mouse strain. That is why we choose to create our knock-in mouse lines conditionally and germline transmission has been achieved for all projects to date. Our publications showcase successful research using Ozgene generated conditional knock-in mice. You can trust that we will strive to achieve the same outcome for you.

  • How are knock-in mice used in research? >

    Knock-in mouse models have several applications in medical research, especially to study and develop treatments for human diseases. Mice with point mutations allow researchers to study the role of these mutations in relation to various human conditions. Reporter knock-in mice enable the tracking of gene expression and promoter activity. Knock-in mice can also be used as models for preclinical drug validation.

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KI publications

Over 400 scientific publications are based on research using Ozgene mice. Over 130 of them feature a conditional or non-conditional knock-in mouse. Some of the knock-in designs also incorporate a knockout of the gene of interest.

Go to papers

KO & KI service

  • Design & construct vector & probes
  • Fully sequence vector & probes
  • Electroporate embryonic stem (ES) cells
  • Screen, expand & confirm ES cells
  • Inject ES cells into goGermline blastocysts
  • Generate & breed goGermline chimeras
  • Genotype germline offspring

Custom breeding

  • Cre breeding
  • FLPe breeding
  • Tissue specific breeding
  • Reserve breeding stock
  • Homozygous lines
  • Colony production
  • Customised breeding

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