Knockout mice

knockout mice

✓ Generation in as little as 20 weeks
✓ Over 300 mouse models published
✓ Using novel goGermline technology

"I selected Ozgene because of the reputation, scientific quality and compatible prices. The best aspects of Ozgene service were professionalism, friendly staff and regular updates on work progress."

- Prof. Dee Harrison-Findik, University of Nebraska, USA

Generate knockout mice with Ozgene

Ozgene is considered the leader in custom designed knockout mice; we have over two decades of experience creating mouse models for pivotal medical research globally. In fact, Dr Koentgen and Dr Suess were the first to develop and publish C57BL/6 knockout mice in 1993.

Our knockout mouse projects now use goGermline, the revolutionary new technology to generate ES cell derived mice fast and efficiently. All of our knockout mouse projects have resulted in germline transmission. This gives us a proven track record, which is evidenced by the multitude of research publications utilising our mouse models.

Ozgene is making continuous improvements on project timelines by stabilising, standardising and refining each individual process using Lean management. Ozgene’s fastest conditional knockout mouse project was recently completed in 20 weeks and our current average timeline to generate conditional knockout mice is 30 weeks.

  • What are knockout mice? >

    Knockout mice have been genetically modified to delete or inactivate a specific gene. The loss of gene function may cause a change in the mouse phenotype. When comparing human and mouse genomes, 80% of the genes have corresponding counterparts (Emes et al. 2003). Therefore, observing the characteristics of knockout phenotype can help researchers understand human gene function and genetic diseases.

  • How does Ozgene create conditional knockout mice? >

    Ozgene uses a conditional approach utilising Cre/loxP technology to generate your knockout model. The Cre recombinase excises DNA between two loxP sites. The loxP sites can be inserted around an essential exon in a gene. The mice are phenotypically wildtype but contain the floxed allele in all tissues. When bred to different Cre mice, tissue specific or inducible knockout mice are generated.

    We continue to rely on the well-published technique of gene targeting via homologous recombination while monitoring and evaluating the reliability of the newer technologies for genome editing, such as CRISPR.

  • What type of research are knockout mice appropriate for? >

    Knockout mice are a valuable scientific method to study gene function. Conditional models enable the study of gene inactivation in a specific cell type, tissue of interest, or at a defined developmental stage. Knockout models are widely used for studying and developing treatments for human diseases, such as cancer, heart disease, diabetes, obesity, arthritis and neurodegenerative diseases. For more information on research using mouse models, please read our newsletter articles featuring client research.
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KO publications

Over 400 scientific publications are based on research using Ozgene mice. Over 300 of them feature a conditional or non-conditional knockout mouse. Some of the knockout designs also incorporate knock-in of a reporter, point mutation or humanisation.

Go to papers

KO & KI service

  • Design & construct vector & probes
  • Fully sequence vector & probes
  • Electroporate embryonic stem (ES) cells
  • Screen, expand & confirm ES cells
  • Inject ES cells into goGermline blastocysts
  • Generate & breed goGermline chimeras
  • Genotype germline offspring

Custom breeding

  • Cre breeding
  • FLPe breeding
  • Tissue specific breeding
  • Reserve breeding stock
  • Homozygous lines
  • Colony production
  • Customised breeding

Get in touch

Australia +61 8 9212 2200

USA +1 617 945 8307