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Compositions and methods for producing genetically modified animals

Compositions and methods for producing genetically modified animals

Compositions and methods for producing genetically modified animals

ABSTRACT

Methods, compositions and non-human animals and parts thereof are disclosed for improving germ line transmission of genetic modifications. More particular, the present invention discloses methods and compositions for producing non-human embryos with a disrupted or disruptable fertility gene, which can be used as hosts for the development of donor pluripotent cells, including genetically modified donor pluripotent cells, into germ cells and gametes. Also disclosed are methods and compositions for producing from such embryos chimeric non-human animals with a disrupted fertility gene and for breeding the chimeric non-human animals with cognate non-human animals that comprise a fertility gene that lacks a disruption to produce non-human animals having substantially all gametes and/or germ cells derived from the donor pluripotent cells. The present invention also discloses non-human gametes, germ cells, embryos and animals for use in the subject methods.

WIPO: WO2015066768A1
Australia: AU2014346342C1
South Korea: KR20160084431A
Canada: CA2928744A1
United States: US20160360736A1
New Zealand: NZ719493A
Singapore: SG11201603352SA
China: CN105899667A
Europe: EP3066204A4
Japan: JP2016539660A
Israel: IL245306D0