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Sequential cloning system

Sequential cloning system

Sequential cloning system

ABSTRACT

This invention discloses a cloning system and more particularly a system for sequentially cloning a plurality of heterologous nucleic acid sequences to assemble a chimeric construct of interest. The cloning system employs a marker sequence, which confers an identifiable characteristic on host cells in which it is contained, to chaperone individual insert nucleic acid sequences into recipient constructs that do not comprise the marker sequence but comprise other nucleic acid sequences for inclusion in the chimeric construct. Recombinant constructs into which one or more insert nucleic acid sequences have been introduced with the chaperone marker sequence are isolated by introducing recombinant constructs into host cells and identifying hosts cells with the identifiable characteristic.

WIPO: WO2006122354A1
Austria: AT545701T
Australia: AU2006246975B2
Canada: CA2608636C
Europe: EP1882036B1
Japan: JP2008539766A
United States: US8202727B2