• Ozgene generates mouse models via gene targeting in ES cells using our proprietary goGermline™ technology, which vastly improves the 3R efficiencies and the timeline for development of gene targeted mouse models.
• We also use various technologies to validate and phenotype your mouse lines e.g. RT-qPCR, Western Blot, ELISA and flow cytometry.
• Ozgene is able to create large, gene-targeted replacement models. This system enables routine generation of mouse models with very large genomic replacements.

Ozgene offers gene targeting on the following backgrounds:

• C57BL/6J
• C57BL/6N
• BALB/c
• FVB/n
• NOD
• Less common strains as required

We generate genetically engineered humanized models where the human gene replaces the mouse gene, for example

• humanizing point mutations,
• humanizing domains, such as extracellular domains,
• humanizing the entire gene,
• humanizing multi-gene loci.

• Knock in mice
• Knock out mice
• Conditional knockout mice (or floxed mice)
• Other transgenic mice

• Predictable Phenotypes: Targeted modifications result in predictable phenotypes, making it easier to understand the role of specific genes in disease and assess the impact of potential drugs.
• Improved Model Validity: By closely mimicking human genetic conditions, targeted transgenic mice provide more accurate models for studying human diseases and drug responses.
• Reduced Risk of Unintended Effects: The precision of targeted integration reduces the risk of unintended genetic effects, ensuring that observed drug responses are due to the intended gene modifications.